BRANCHING GLYCOSYLTRANSFERASE FROM RHODOTHERMUS OBAMENSIS EXPRESSED IN BACILLUS SUBTILIS
New specifications prepared at the 71st JECFA (2009) and published in FAO JECFA Monographs7 (2009). An ADI "not specified" was established at the 71st JECFA (2009).
SYNONYMS 1,4-α-glucan branching enzyme; amylo-(1,4→1,6)-transglycosylase; α–glucan-branching glycosyltransferase; branching enzyme; Q-enzyme
SOURCES Branching glycosyltransferase is produced by submerged fedbatch fermentation of a genetically modified strain of Bacillus subtilis which contains a gene coding for branching glycosyltransferase from Rhodothermus obamensis. The enzyme is recovered from the fermentation broth. The recovery process includes the separation of cellular biomass, concentration by ultrafiltration and/or evaporation, and germ filtration. The final product is formulated using food-grade stabilizing and preserving agents and is standardized to the desired activity.
Active principles Branching glycosyltransferase
Systematic names and numbers 1,4-α-D-glucan:1,4-α-D-glucan 6-α-D-(1,4-α-D-glucano)-transferase; EC 2.4.1.18; CAS No. 9001-97-2
Reactions catalysed Transfers a segment of a 1,4-α-D-glucan chain to a primary hydroxy group in a similar glucan chain to create 1,6-α-linkages
Secondary enzyme activities
No significant levels of secondary enzyme activities
DESCRIPTION Light brown liquid
FUNCTIONAL USES Enzyme preparation.
Used in starch processing to obtain modified starch with improved functional properties
GENERAL SPECIFICATIONS
Must conform to the latest edition of the JECFA General Specifications and Conside
