α-ACETOLACTATE DECARBOXYLASE from BACILLUS BREVIS expressed in BACILLUS SUBTILIS
Prepared at the 53rd JECFA (1999) and published in FNP Add 7 (1999), superseding specifications prepared at the 51st JECFA (1998),
published in FNP 52 Add 6 (1998). ADI "not specified" established at the 51st JECFA in 1998.
SOURCES Produced extracellularly by submerged fermentation of Bacillus subtilis, which through recombinant DNA techniques contains the gene for alphadecarboxylase from Bacillus brevis. The strain of Bacillus subtilis is nonpathogenic and non-toxicogenic (e. g. UW193 (a dal-transformant strain
of ToC46) ). When fermentation is complete, the broth is filtered then stabilised before further filtration.
Active principles alpha-Acetolactate decarboxylase
Systematic names and numbers (S)-2-Hydroxy-2-methyl-oxobutanoate carboxylase (EC 4.1.1.5) Reactions catalysed Decarboxylation of alpha-acetolactate to acetoin DESCRIPTION Brown liquid containing the active enzyme; Total Organic Solids approx. 2%
FUNCTIONAL USES Enzyme preparation
Used in the brewing of beer and fermentation step of alcohol production
GENERAL SPECIFICATIONS Must conform to the General Specifications for Enzyme Preparations
Used in Food Processing (Volume Introduction)
