BROMELAIN
Prepared at the 15th JECFA (1971), published in NMRS 50B (1972) and in FNP 52 (1992). An ADI 'not limited' was established at the 15th JECFA (1971) SYNONYMS INS No. 1101(iii)
SOURCES Purified proteolytic substances derived from Ananas comosus and Ananas bracteatus (L).
Active principles Bromelain (cystein proteinase)
Systematic names and numbers None (EC 3.4.22.4)
Reactions catalyzed The enzyme hydrolyzes polypeptides, amides and esters, especially at linkages involving basic amino acids, or leucine or glycine, yielding peptides of lower molecular weight.
DESCRIPTION White to light tan amorphous powder; soluble in water, the solutions being colourless to light yellow and somewhat opalescent; practically insoluble in alcohol, chloroform and ether
FUNCTIONAL USES Enzyme preparation
Used in the chillproofing of beer, tenderizing of meat, preparation of precooked cereals, and production of protein hydrolysates.
GENERAL SPECIFICATIONS
Must conform to the General Specifications for Enzyme Preparations used in Food Processing (see Volume Introduction)