Cellulase from Penicillium funiculosum

Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 31st JECFA (1987) and published in FNP 38 (1988) and in FNP 52 (1992). No ADI was allocated at the 31st JECFA (1987).
SOURCES Produced by the controlled fermentation of non-toxicogenic and nonpathogenic strains of Penicillium funiculosum and isolated from the growth medium.
Active principles Cellulase (endo-1,4-beta-glucanase)
Endo-1,3(4)-beta-glucanase
Endo-1,4- beta-xylanase
Systematic names and numbers
1,4-(1,3; 1,4)- beta-D-Glucan-4-glucanohydrolase (EC 3.2.1.4)
1,3-(1,3; 1,4)- beta-D-Glucan-3(4)-glucanohydrolase (EC 3.2.1.6)
1,4- beta-D-xylan xylohydrolase (EC 3.2.1.8)
Reactions catalyzed Hydrolyzes 1,4-beta-glucan linkages in polysaccharides such as cellulose,
yielding beta-dextrins.
Secondary enzyme activities
alpha-N-Arabinofuranosidase
Cellulose 1,4- beta-cellobiosidase
beta-glucosidase
Xylan 1,4-beta-xylosidase
DESCRIPTION Typically off-white to tan amorphous powders, or liquids dispersed in foodgrade carriers or diluents; soluble in water; practically insoluble in ethanol and ether.
FUNCTIONAL USES Enzyme preparation. Used in the preparation of fruit juices, wine, beer and vegetable oils
GENERAL SPECIFICATIONS
Must conform to the General Specifications for Enzyme Preparations Used in Food Processing (see Volume Introduction)