ß-GLUCANASE from ASPERGILLUS NIGER, var.
Prepared at the 41st JECFA (1993), published in FNP 52 Add 2 (1993) superseding specifications prepared at the 31st JECFA (1987), published
in FNP 38 (1988) and in FNP 5 (1992). An ADI 'not specified' was established at the 35th JECFA (1989)
SYNONYMS Endo-1,3-ß-glucanase
SOURCES Produced by the controlled fermentation of Aspergillus niger, var. and isolated from the medium.
Active principles Endo-1,3(4)-ß-glucanase
Systematic names and numbers
1,3-(1,3; 1,4)-ß-D-Glucan-3(4)- glucanohydrolase (EC 3.2.1.6)
Reactions catalyzed ß-glucanase hydrolyzes 1,3-ß- and 1,4-ß -glucosidic linkages in ß-Dglucans yielding oligosaccharides and glucose
Secondary enzyme activities
Hemicellulase
Pectinase (EC 3.2.1.15)
DESCRIPTION Off-white to tan amorphous powders or tan to dark-brown liquids dispersed in food-grade carriers and diluents, and may contain stabilizers and preservatives; soluble in water and practically insoluble in ethanol, chloroform and ether
FUNCTIONAL USES Enzyme preparation
Used in the preparation of fruit juices and beer; manufacture of cheese
GENERAL SPECIFICATIONS
Must conform to the General Specifications for Enzyme Preparations used in Food Processing (see Volume Introduction)
