CELLULASE from TRICHODERMA REESEI TENTATIVE
Prepared at the 31st JECFA (1987), published in FNP 38 (1988) and in FNP 52 (1992). An ADI 'not specified' was established at the 39th JECFA
(1992) Information required on the applicability of Method of Assay for Cellulase
SOURCES Commercial enzyme preparations are produced by the controlled fermentation of Tricoderma reeser and isolated from the medium
Active principles
1. Cellulase (endo-1,4-ß-glucanase)
2. Exo-1,4-ß-D-glucosidase (glucan-1,4-ß-glucosidase)
3. Exo-cellobiohydrolase (cellulose 1,4-ß-cellobiosidase)
4. ß-glucanase
Systematic names and numbers
1. 1,4-(1,3; 1,4)-ß-D-Glucan-4-glucanohydrolase (EC 3.2.1.4)
2. 1,4-ß-D-Glucoside glucohydrolase (EC 3.2.1.74)
3. 1,4-ß-D-Glucan cellobiohydrolase (EC 3.2.1.91)
4. 1,3-(1,3; 1,4)-ß-D-glucan-3(4)-glucanohydrolase (EC 3.2.1.6)
Reactions catalyzed The enzyme preparations hydrolyze 1,4-ß-glucan linkages in such polysaccharides as cellulose, yielding ß-dextrins.
Secondary enzyme activities Xylanase (EC 3.2.1.32)
Beta-glucosidase (EC 3.2.1.21)
DESCRIPTION Off-white to tan amorphous powders, or liquids that may be dispersed in food-grade diluents and carriers; soluble in water but practically insoluble in ethanol, chloroform and ether
FUNCTIONAL USES Enzyme preparation
Used in the preparation of fruit juices, wine, beer and vegetable oils
GENERAL SPECIFICATIONS
Must conform to the General Specifications for Enzyme Preparations used in Food Processing (see Volume Introduction)
