β-GLUCANASE from TRICHODERMA HARZIANUM
Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding specifications prepared at the 39th JECFA (1992)
and published in FNP 52 Add 1 (1992). An ADI “Not specified” was established at the 39th JECFA (1992).
SOURCES Produced by the controlled fermentation of non-pathogenic and nontoxicogenic strains of Trichoderma harzianum (classification by Rifai M.A.,
Mycological Papers, No. 116, pages 1-56, 1969) and isolated from the growth medium.
Active principles Endo-1,3-beta-glucanase (synonym: laminarinase) Exo-1,3-beta-glucanase
Systematic names and numbers
1,3-(1,3; 1,4)-beta-D-glucan 3(4) glucanohydrolase (EC 3.2.1.6; C.A.S. No. 62213-14-3)
Glucan 1,3-beta-glucosidase (EC 3.2.1.58; C.A.S. No. 9073-49-8)
Reactions catalyzed Hydrolyzes beta-1,3 or beta-1,4 linkages in 1,3 (1,4)-beta-D-glucans yielding glucose.
Secondary enzyme activities
Hemicellulase
Cellulase (1,4-[1,3;1,4]-beta-D-Glucan 4-glucano-hydrolase); (EC 3.2.1.4;
C.A.S. No. 9012-54-8)
Pectinase (Poly (1,4-alpha-D-galacturonide) glycanohydrolase); (EC
3.2.1.15; C.A.S. No. 9032-75-1)
DESCRIPTION Typically off-white to tan amorphous powders or tan to dark-brown liquids.
These products are concentrated and standardised with food-grade diluents or carriers such as maltodextrin, starch or glucose to obtain
commercial preparations. Soluble in water and practically insoluble in ethanol and ether.
FUNCTIONAL USES Enzyme preparation Used in the preparation of fruit juices, wine, beer and vegetable oils
