MIXED XYLANASE, β-GLUCANASE ENZYME PREPARATION, produced by a strain of HUMICOLA INSOLENS
New specifications prepared at the 61st JECFA (2003) and published in FNP 52 Add 11 (2003). An ADI “not specified” was established. SOURCES Produced by submerged fermentation of a non-pathogenic and non-toxigenic strain of Humicola insolens. The manufacturing process is composed of a fermentation process, a purification process, a formulation process and finally a quality control of the finished product. The cell mass and other solids are separated from the broth by vacuum drum filtration or centrifugation. Ultrafiltration and/or evaporation are applied for concentration and further purification. Residual production strain microorganisms are removed by germ filtration.
Active principles beta-Glucanase and xylanase
Systematic names and numbers
beta-Glucanase: 1,3-(1,3;1,4)-beta-D-glucan 3(4)-glucanohydrolase
EC number: 3.2.1.6
CAS number: 62213-14-3
Xylanase: 1,4-beta-D-xylan xylanohydrolase
EC number: 3.2.1.8
CAS number: 9025-57-4
Reactions catalysed Endohydrolysis of 1,3- or 1,4-linkages in beta-D-glucans
Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans
Secondary enzyme activitiesCellulase CAS No. 9012-54-8
Hemicellulase CAS No. 9025-56-3
Pentosanase CAS No. 9068-42-2
Arabinase CAS No. 75432-96-1
DESCRIPTION Brown liquid.
The enzyme concentrate as manufactured is stabilized by the addition of sorbitol, glycerol, and potassium sorbate.
