PULLULANASE FROM BACILLUS DERAMIFICANS EXPRESSED IN BACILLUS LICHENIFORMIS
New specifications prepared at the 74th JECFA (2011) and published in FAO JECFA Monographs 11 (2011). An ADI "not specified" was
established at the 74th JECFA (2011).
SYNONYMS Pullulan α-1,6-glucanohydrolase; amylopectin 6-glucanohydrolase, bacterial debranching enzyme; α–dextrin endo-1,6-glucosidase;
debranching enzyme; R-enzyme
SOURCES Pullulanase is produced by submerged fed-batch fermentation of a genetically modified strain of Bacillus licheniformis which contains a
gene coding for pullulanase from Bacillus deramificans. The enzyme is recovered from the fermentation broth. The recovery process includes the separation of cellular biomass and concentration by ultrafiltration. The final product is formulated using food-grade stabilizing and preserving agents and is standardized to the desired activity.
Active principles Pullulanase
Systematic names and numbers
Pullulan 6-α-glucanohydrolase; EC 3.2.1.41; CAS No. 9075-68-7
Reactions catalysed Hydrolysis of (1→6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen
Secondary enzyme activities No significant levels of secondary enzyme activities
DESCRIPTION Amber liquid
FUNCTIONAL USES Enzyme preparation.
Used in the hydrolysis of carbohydrates in the manufacture of starch hydrolysates (maltodextrins, maltose and glucose), high fructose corn syrup, beer, and potable alcohol.
