Phospholipase C expressed in Pichia pastoris

New specifications prepared at the 69th JECFA (2008), published in FAO JECFA Monographs 5 (2008). An ADI "not specified" was
established at the 69th JECFA (2008).
SYNONYMS Phospholipase C; lecithinase C; lipophosphodiesterase C; phosphatidase C
SOURCES Phospholipase C is produced by submerged fed-batch fermentation of a genetically modified strain of Pichia pastoris which contains the
phospholipase C gene derived from a soil sample. The enzyme is recovered from the fermentation broth. The recovery process includes the separation of cellular biomass, clarification, ultrafiltration, diafiltration, and polish filtration. The final product is formulated using food-grade stabilizing and preserving agents and is standardized to the desired activity.
Active principles Phospholipase C
Systematic names and numbers
Phosphatidylcholine cholinephosphohydrolase; EC 3.1.4.3; CAS No. 9001-86-9
Reactions catalysed Hydrolysis of phosphodiester bonds at the sn-3 position in glycerophospholipids including phosphatidylcholine,  phosphatidylethanolamine, and phosphatidylserine to yield 1,2-diacylglycerol and the corresponding phosphate esters
Secondary enzyme activities
No significant levels of secondary enzyme activities.
DESCRIPTION Yellow to brown liquid
FUNCTIONAL USES Enzyme preparation.
Used in refining vegetable oils intended for human consumption.