AOAC Official Method 941.05 Sterols in Macaroni Products

Bromination Method
First Action 1941
Final Action
(As cholesterol.)
Determine sterols in unsaponifiable matter as in 941.09F (see 34.1.09). However, to unsaponifiable matter from egg-free products or from any product containing <0.23% unsaponifiable matter (as-is basis), add 10 mg cholesterol before applying cholesterol method and correct result accordingly. (For the added cholesterol use highest quality obtainable [mp ³147°C] and test its purity by carrying 20 mg through determination.)

References: JAOAC 24, 75, 143(1941); 25, 83, 639(1942).
CAS-57-88-5 (cholesterol)