AOAC Official Method 974.38 Clostridium perfringens in Foods a-Toxin Estimation Method
First Action 1974
Final Action 1979
(Applicable to examination of outbreak foods in which presence of large numbers of vegetative cells are suspected but which may no longer be viable.)
A. Apparatus
(a) Centrifuge.High-speed, preferably refrigerated, with 250 mL bottles.
(b) Seitz filter.100250 mL with sterilizing filter pads.
(c) High-speed blender.With blending vessels.
(d) Vacuum flask.Sidearm 1 L Erlenmeyer fitted with 1-hole rubber stopper to receive 200mmglass tubing with 125 cm of 6mm od (3 mm id) rubber tubing attached.
(e) Tubing.Stainless steel thin wall (No. 9 surgical), 3 (od) ´180 mm (Tubesales, 175 Tubeway St, Forrest Park, GA 30297,USA).
(f) Dialysis tubing.1.21 in. (30.7 mm) flat width (Fisher Scientific Co., 15 Jet View Dr, Rochester, NY 14624, USA).
