AOAC Official Method 940.03 Chlorophyll in Plants Photoelectric Colorimetric Method for Total Chlorophyll Only
First Action 1940
Final Action
A. Apparatus
(a) Mortar and pestle.—Deep glass mortar ca 10 cm id with well-defined lip.
(b) Photoelectric colorimeter.—Calibrate for chlorophyll, using plant extract as in C and light filters with maximum T near 660 nm. (Combination of Kopp Glass Works filters K2408 and K3965 is suitable.)
(c) Wash bottles.—Type fitted with rubber bulb, permitting operation with one hand.
(d) High-speed blender.—Waring Blendor, or equivalent.
B. Reagents
(a) Acetone.—(1) Undiluted acetone and (2) 85% aqueous solution by volume. Commercial acetone, technical grade, is satisfactory.
(b) Quartz sand.—Acid-washed and dried.
