AOAC Official Method 938.04 Carotenoids in Macaroni Products
Colorimetric Method
First Action 1938
Final Action
(Applicable only to determination of carotenoids added for coloring purposes.)
A. Reagents
(a) Alcoholic potassium hydroxide solution.—Dissolve 10 g KOH in 100 mL alcohol by warming on steam bath.
(b) Methanol.—92%. 8 mL H2O + 92 mL absolute methanol.
(c) Adsorption mixture.—Mix equal portions by volume of activated magnesia (Micron brand No. 2641, 2642, or Adsorptive Magnesia No. S-120, Fisher Scientific Co.) and diatomaceous earth (Hyflo Super-Cel, Celite Corp.).
