AOAC Official Method 936.14 Vitamin D in Milk, Vitamin Preparations, and Feed Concentrates Rat Bioassay
First Action 1936
Final Action
(Applicable to all food products; not applicable to products offered for poultry feeding.)
A. Definitions
Assay group means group of rats to which test sample is administered during assay period. Test sample means product under examination for vitamin D potency. Test solution means solution of product in oil prepared for feeding after saponification. Assay period means interval in life of rat between last day of depletion period and eighth or 11th day thereafter. Assemble means procedure by which rats are selected and assigned to groups for purposes of feeding, care, and observation. Daily means each of first 6 or 8 days of assay period.
Depletion period means interval in life of rat between last day of preliminary period and first day of assay period. Dose means amount of reference oil or of assay milk or other supplement to be fed daily to rat during assay period. Feed means make readily available to rat or administer to rat by mouth. Group means 7 or more rats maintained on same required dietary regime during assay period. Preliminary period means interval in life of rat between 7th day after birth and first day of depletion period.
