Pullulanase from Klebsiella aerogenes

Prepared at the 25th JECFA (1981), published in FNP 19 (1981) and in FNP 52 (1992). No ADI was allocated at the 25th JECFA (1981)
SYNONYMS R-enzyme, limit dextrinase, debranching enzyme
SOURCES Produced by the controlled fermentation of Klebsiella aerogenes
Active principles Pullulanase (alpha-dextrin endo-1,6-alpha-glucosidase, amylopectin 6-glucanohydrolase)
Systematic names and numbers
alpha-Dextrin 6-glucanohydrolase (EC 3.2.1.41)
Reactions catalyzed The enzyme preparations hydrolyze 1,6-alpha-glucosidic linkages in pullulan, and the alpha- and ß-amylase limit dextrins of amylopectin and glycogen, yielding mainly maltotriose and maltose.
Secondary enzyme activities alpha-amylase (EC 3.2.1.1)
Microbial serine proteinase (EC 3.4.21.14)
Microbial aspartic proteinase (EC 3.4.23.6)
DESCRIPTION Tan, fine powders or off-white liquids; soluble in water; practically insoluble in ethanol, chloroform and ether
FUNCTIONAL USES Enzyme preparation
Used in the preparation of and/or use in sugar and honey, cereal and starch and beverages
GENERAL SPECIFICATIONS
Must conform to the General Specifications for Enzyme Preparations used in Food Processing (see Volume Introduction)