Rennet from Rhizomucor species

Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 15th JECFA
(1971) and published in NMRS 50B (1972) and in FNP 52 (1992). An ADI "Not specified" was established at the 18th JECFA (1974).
SOURCES Produced by the controlled fermentation of non-pathogenic and nontoxicogenic strains of Rhizomucor miehei or Rhizomucor pusillus.
Active principle Microbial aspartic proteinase (acid protease)
Systematic name and number
EC 3.4.23.23; C.A.S. No. 9073-79-4
Reactions catalyzed Hydrolyzes polypeptides, e.g. casein, yielding peptides of lower molecular weight.
DESCRIPTION White to tan amorphous powders; soluble in water, the solution usually being light yellow in colour; practically insoluble in ethanol and ether.
FUNCTIONAL USES Enzyme preparation
Used in clotting of milk for cheese production
GENERAL SPECIFICATIONS
Must conform to the “General Specifications for Enzyme Preparations
Used in Food Processing” (see Volume Introduction)