AOAC Official Method 938.18 Glucose in Cacao Products Zerban-Sattler Method
First Action 1938
A. Reagents
(a) Soxhlet modification of Fehling copper solution.—See 923.09A(a) (see 44.1.15).
(b) Sodium acetate solution.—Dissolve 500 g NaCH3COO×3H2O in ca 800 mL hot H2O, cool, and dilute to 1 L.
(c) Potassium iodide–iodate solution.—Dissolve 5.4 g KlO3 and 60 g KI in H2O, add 0.25 g NaOH dissolved in little H2O, and dilute to 1 L.
(d) Sulfuric acid.—Approximately1M.Dilute 57mLH2SO4 to1L.
(e) Saturated potassium oxalate solution.—Dissolve 165 g K2C2O4×H2O in 500 mL hot H2O, and cool.
(f) Sodium thiosulfate standard solution.—0.1M Use standard solution, 942.27 (see A.1.13). 1 mL 0.1M Na2S2O3 = 6.354 mg Cu.
(g) Sugar solution.—Dissolve amount of test sample containing ca 10 g solids in H2O and dilute to 1 L.
